Overexpressing TDP-43 inhibits NF-κB activity by competing for the nuclear transporter.

<p><b>A</b>. Cells were transfected with wild type TDP-43 expressing plasmids (tagged with mCherry) or mCherry empty plasmid (control) and were then treated with 10ng/ml TNF-α for 30 minutes. Cell lysates were subjected to coimmunoprecipitation and western blot for the presence of p65 and TDP-43. 10% cell lysate were reserved as input. p65, TDP-43 and importin α3 (KPNA4) were used to detect input. n = 3 repeats. <b>B</b>. The relative density of p65 as shown in A (IP), *p<0.05. <b>C</b>. The relative density of wild type TDP-43 as shown in A (IP), *p<0.05.