TDP-43 regulates levels of misfolded proteins.

<p>(A) Ectopic expression of TDP-43 increases the aggregation formation of SOD1 G85R. HEK293T cells were co-transfected with plasmids expressing SOD1 G85R and WT TDP-43. The cells were detergent-extracted to separate soluble proteins (unaggregated and oligomers) and insoluble aggregates. (B) Knockdown of TDP-43 suppressed the aggregation of SOD1 G85R. HEK293T cells were co-transfected with plasmids expressing SOD1 G85R and shRNA against TDP-43 or a scrambled control. The cells were detergent-extracted as described and subjected to western blotting analysis.