ORFV002 prevents NF-κB-p65 Ser276 phosphorylation.

<p>A. OFTu cells were co-transfected with plasmid pNF-κB-p65 and either pEGFP-N1 or pORFV002-EGFP. At 24 hours post transfection, cells were treated with 20 ng/ml of TNF-α for 0, 30 and 60 minutes and harvested at the indicated times. Nuclear protein fractions were extracted. Protein extracts (50 μg) were resolved by SDS-PAGE, blotted, and probed with antibodies against p-NF-κB-p65-Ser²⁷⁶ (top), p-NF-κB-p65-Ser⁵³⁶ (middle), or histone H3 (bottom). B. Densitometry of p-NF-κB-p65 Ser²⁷⁶ and Ser⁵³⁶ bands normalized to loading control histone H3 (**, P<0.005). The results are representative of three independent experiments.