TNF-α increases cytoplasmic localization of TDP-43 in NSC-34 cells.

<p>Non-transfected NSC-34 cells and hTDP-43WT-HA stably-transfected NSC-34 cells were treated with recombinant TNF-α at a concentration of 10 ng/mL for 6h. Non-TNF treated cells served as controls. Following treatment, the subcellular distribution of TDP-43 was determined by Western blot analysis. Immunoblotting with polyclonal TDP-43 antibody showed increased cytoplasmic TDP-43 in TNF-α-treated NSC-34 cells (left first panel) and hTDP-43WT-HA stably transfected cells (right first panel). Similarly, immunoblotting with monoclonal TDP-43 antibody (specific for human TDP-43) showed increased levels of cytoplasmic TDP-43 in TNF-α-treated hTDP-43WT-HA stably-transfected NSC-34 cells (2nd left panel). Immunoblots were reprobed with P84 antibody (nuclear marker) and GAPDH antibody (cytoplasmic marker).