MFHAS1 induces phosphorylation of JNK and p38.

<p>(A) RAW 264.7 macrophages were transfected with HIS-MFHAS1 or pCDH empty vector plasmid, and 24 h post-transfected cells were treated with 10 ng/mL Pam3CSK4 for 0, 1, 3, 5, 30, 60, 90 min, respectively. Cells were collected and the protein levels of pJNK, JNK, pp38, p38, pERK, ERK, HIS-MFHAS1 and GAPDH were examined by western blotting. (B) Quantified data of the pJNK, pp38, pERK levels. Levels of pJNK, pp38, pERK respectively normalized to JNK, p38, ERK levels were shown.