Activation of p38/JNK (A) and p-IκBα (B) in peritoneal murine macrophages (C57BL/6, TLR2 -/- and TLR4 -/-) by <i>L</i>. <i>amazonensis</i> LPGs (PH8 and Josefa).
- Paula M. Nogueira (2935749)
- Rafael R. Assis (2935752)
- Ana C. Torrecilhas (466115)
- Elvira M. Saraiva (308686)
- Natália L. Pessoa (2935746)
- Marco A. Campos (224562)
- Eric F. Marialva (2935761)
- Cláudia M. Ríos-Velasquez (2935767)
- Felipe A. Pessoa (2935758)
- Nágila F. Secundino (2935773)
- Jerônimo N. Rugani (2935770)
- Elsa Nieves (2935755)
- Salvatore J. Turco (237607)
- Maria N. Melo (220015)
- Rodrigo P. Soares (2935764)
Publication date
August 2016
DOIAbstract
<p>Macrophages were stimulated for 5, 15, 30, 45 and 60 min with 10 μg/mL of LPG from <i>L</i>. <i>amazonensis</i> PH8 and Josefa strains. Dually phosphorylated MAPKs (p38 and JNK) and p-IκBα were detected by Western blot analysis. C- = negative control; C+ = <i>E</i>. <i>coli</i> extract, positive control (100 ng/mL, 45 min). Total p38 content was used as the normalizing protein.</p
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