Analysis of the protein-protein interaction network of Hpn and Hpn-2 by <i>in vivo</i> BACTH.

<p>(A-C) the tables represent the results of the β-galactosidase activity measurements on <i>E</i>. <i>coli</i> strains containing the pair-wise combinations of the different constructs. T18 and T25 correspond to the two fragments of the adenylate cyclase and the protein extremity at which the fusion was made (N_ter or C_ter) is shown. Panel A: interaction between Hpn, Hpn∆Cter, Hpn-2 and Hpn-2∆Cter. Panel B and C: interaction of Hpn, Hpn∆Cter, Hpn-2 and Hpn-2∆Cter with UreA, HypA and HypB. Red squares correspond to β-galactosidase activity that are > 3,000 U, orange squares between 2,000 and 3,000 U, yellow squares between 500 and 2,000 U and light yellow squares, between 240 and 500 U. None of the potential false positive interactions was presented (interactions of pUT18/pKNT25(Hpn) and pUT18(Hpn)/pKNT25 with ß-galactosidase activity below 600 units). Black squares represent combinations that lead to a lethal phenotype. The β-galactosidase activity values for each strain and the controls are indicated in <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1005312#ppat.1005312.s006" target="_blank">S2 Table</a>. (D) Schematic representation of the protein-protein interaction network of Hpn and Hpn-2. Thick arrows correspond to strong interactions, thin arrows to moderately strong interactions, according to the ß-galactosidase. * Indicates that this interaction was established before [<a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1005312#ppat.1005312.ref053" target="_blank">53</a>].