Two-hybrid analysis of interactions between Pxa2_NBD-CT (or Pxa2_NBD) and Pxa1_NBD-CT (or Pxa1_NBD).
- Cheng-Yi Chuang (614833)
- Ling-Yun Chen (614834)
- Ru-Huei Fu (308319)
- Shih-Ming Chen (614835)
- Ming-Hua Ho (614836)
- Jie-Mau Huang (614837)
- Chia-Chi Hsu (614838)
- Chien-Cheng Wang (614839)
- Meng-Shian Chen (614840)
- Rong-Tzong Tsai (385376)
Publication date
August 2014
DOIAbstract
<p>The EGY48 (pSH18-34) reporter strains that express the indicated hybrid proteins (with the Pxa2 fragment fused to LexA BD and the Pxa1 fragment fused to Gal4 AD) were analyzed for β-galactosidase activity on an X-gal plate. Plates were incubated for 16 h at 30°C. Blue indicates the existence of a protein-protein interaction between hybrid proteins.</p
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