Quantification of β-galactosidase in Y2H experiment.

<p>The upper and lower panels show the β-galactosidase activity for Pil protein interactions in Y2H experiments from <i>M. xanthus</i> and <i>T. thermophilus</i>, respectively. The values for β-galactosidase activity were the average of three independent experiments and samples in each experiment were analyzed in triplicate. See <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0070144#pone-0070144-g004" target="_blank">Figure 4</a> for protein designations under each panel. The bars for the vector controls are shaded for comparison. See text and <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0070144#s2" target="_blank">Materials and Methods</a> for more details.