Analysis of the interacting properties of different domains of R6 by yeast two-hybrid analyses.

<p>Upper panels: yeast THY-AP4 strain was transformed with plasmids pBTM-R6 wt (LexA-R6), pBTM-R6 RARA and pBTM-R6 RAHA (A), pBTM-R6 WDNAD, and pBTM-WANNA (B) or pBTM-R6 S25A and pBTM-R6 S74A (C) and with pACT2 (GAD), pACT2-PP1α (GAD-PP1α), pACT2-laforin (GAD-laforin) or pACT2-14-3-3ε (GAD-14-3-3ε). Protein interaction was estimated by measuring the β-galactosidase activity. Values correspond to means from at least 6 different transformants (bars indicate standard deviation). Lower panels: protein expression in yeast transformants was analyzed by Western blotting using anti-HA antibodies (for the GAD-fusions) and anti-LexA (for the LexA-fusions) in several transformants from each condition. A representative western blot of some of these transformants is shown.