Yeast-two-hybrid experiments testing interactions between human FIGNL1, FLIP, RAD51 and DMC1 proteins.

<p>Proteins of interest were fused with Gal4 DNA binding domain (BD, left) and with Gal4 activation domain (AD, top), respectively, and co-expressed in yeast cells. Full-length and truncated protein are schematically represented. For each combination, serial dilutions of yeast cells were spotted on non-selective medium (-LW), moderately selective media (-LWH) and more selective media (-LWHA). ++: Growth on both LWH and LWHA, interpreted as strong interaction. +: Growth on LWH and not on LWHA, interpreted as weak interaction. +*: Growth on LWH but cannot be interpreted as positive interaction because of auto-activation of one of the construct. Growth on neither LWH nor LWHA. n.d. Not determined. Pictures of yeasts are shown in <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1007317#pgen.1007317.s002" target="_blank">S2 Fig</a>.