Gene expression analysis of pro-inflammatory proteins with real-time PCR.

<p>Relative mRNA expression of selected pro-inflammatory proteins was analysed in real-time PCR with gene-specific primers in whole mouse brain hemispheres of adult male wild-type C57BL/6N mice treated with either MT-II <i>i</i>.<i>p</i>. only without MCAO (MT-II-treated; n = 3), in mice at 72h after 30min MCAO that were treated with MT-II <i>i</i>.<i>p</i>. (n = 4), and in mice at 72h after 30min MCAO that were treated with vehicle only (n = 6). cDNA was separately isolated from each hemisphere. Relative Expression is presented as fold changes, calculated with the ΔΔCt-Method, normalized to HPRT mRNA expression and related to the non-ischemic/contralateral hemispheres as references. TNFα induction after MCAO is significantly reduced in mice that were treated with MT-II <i>i</i>.<i>p</i> when compared to vehicle-only treated mice (*p < 0.05, students one-side t-test). Data are shown as relative mRNA expression of each gene and is calculated from two technical replications of n = 3 to 6 mice for each condition (values are given as mean ± SEM).