<i>STAT1</i>^<i>-/-</i> mice have lower oxygen consumption.

<p>Oxygen consumption was measured from isolated liver mitochondria of male mice either fed or fasted using substrates that donate to specific sites in the ETC a. Glutamate-malate for Complex I, b. Succinate (+rotenone) for complex II, and c. TMPD-ascorbate (+rotenone) for Complex IV. Significance of p < 0.05 was determined with two-way ANOVA + Holm Sidak’s post test, n = 6, 8–10 week old male mice d. Amounts of UCP2 mRNA levels were measured in male mice either fed or fasted using qRT-PCR and quantitated relative to tubulin in livers. Values were normalized to <i>STAT1</i>^<i>+/+</i> fed. Significance of p < 0.05 were determined by two-way ANOVA + Holm Sidak’s post test, n = 4, 8–10 week old male mice.