Diminished skeletal muscle mitochondrial respiratory capacity in DTG mice.

<p>(A–C) <i>Ex vivo</i> mitochondrial function assessed by measuring oxygen consumption of permeabilized muscle fibers of <i>Soleus</i> muscle from 17- to 23-wk-old WT, DN-AMPKα2, UCP1-TG and DTG mice; (A) upon substrates only (MO, malate + octanoylcarnitine, state 2 respiration), (B) ADP-stimulated respiration (state 3 respiration) fuelled by different substrates for complex I (MOPG, MO + pyruvate + glutamate) and complex II (MOPGS, MOPG + succinate), and (C) maximal uncoupled respiration (state U) after addition of the chemical uncoupler FCCP (n = 6–8 per group). (D) Citrate synthase (CS) activity in <i>Quadriceps</i> muscle of same mice as indicator of total mitochondrial capacity (n = 5–6 per group). Means with different letters are significantly different (1way ANOVA and Bonferroni's multiple comparisons test, p<0.05).