Assembly efficiency and reaction count under different conditions.

<p>(a) The assembly efficiency and fragment count to assemble a 2000 bp gene using different fragment sizes. (b) The assembly reactions required to assemble sequences up to a million bps from 200 bp fragments under different Gibson Assembly capacities up to 30 fragments at once. In both figures the junction length between fragments is fixed at 20 bps and it is assumed that any sequence to assemble can be evenly divided by the fragments.