Assembly of the gene library.

<p>(A) Gel-eluted fragments from C-fragment libraries after restriction digestion (10% PAGE). The randomized fragments are marked in red; the brackets indicate the C-fragments, participating in one ligation assembly. Marker: 50 bp ladder (Thermo Scientific) (B) Ligation assembly of B1-fragment prior to gel elution. The full-length ligation product is indicated with an arrow. The length of the assembled B1 fragment with flanking BsaI restriction sites comprises 208 bp. Marker: 50 bp ladder (Thermo Scientific) (C) Gel-eluted fragments from B-fragment libraries. The length of the B1 fragment, liberated from the plasmid vector after restriction digestion comprises 182 bp. (D) Gene library ligation assembly. The full-length ligation product is indicated with an arrow. Marker: 1 kb ladder (Thermo Scientific).