<p>(A) Cloning efficiency of the MXS-chaining approach measured as the fraction of positive recombinant plasmids resulting from MXS-chaining ligation reactions. (B) Dependence of the cloning efficiency on sizes of MX-digested vectors and MS-digested inserts. Data from 400 different MXS-chaining ligation reactions is shown.</p
a<p>The competent cells [BL21(DE3)RIL] used had efficiency of 1×10<sup>8</sup> transformants/µg supe...
<div><p>A robust method for the <i>in vivo</i> cloning of large gene clusters was developed based on...
<p>(<b>A</b>) Schematic of SOC-mediated cycled-ligation assembly of multiple inserts directly and se...
a<p>The sum of insert length and template plasmid length does not automatically equal the product le...
<p>(A) The p3B plasmid was linearized by PCR with primers p3Bf and p3Br. In parallel, the kanMX cass...
<p>(A) MluI (M), XhoI (X) and SalI (S) recognition sites are highlighted in green, blue and red, res...
<p>All reaction mixtures contained 1 mM ATP and 5 Weiss unit T4 DNA ligase (Fermentas) and incubated...
WOS: 000089634000029PubMed ID: 11310983This study compares a number of parameters that are important...
PCR fragments and linear vectors containing overlapping ends are easily assembled into a propagative...
<p>(<b>A</b>) Identification of positive and negative colonies by fluorescence. The circled colonies...
To research the effects of vector quantity and competence on the positive cloning rate, with a known...
<p>The number of colonies containing the correct constructs was counted for each experiment and the ...
The polymerase chain reaction (PCR) has found wide application in biochemistry and molecular biology...
A robust method for the in vivo cloning of large gene clusters was developed based on homologous rec...
(A) Transformation Efficiency comparison against the total concentration of DNA transformed for the ...
a<p>The competent cells [BL21(DE3)RIL] used had efficiency of 1×10<sup>8</sup> transformants/µg supe...
<div><p>A robust method for the <i>in vivo</i> cloning of large gene clusters was developed based on...
<p>(<b>A</b>) Schematic of SOC-mediated cycled-ligation assembly of multiple inserts directly and se...
a<p>The sum of insert length and template plasmid length does not automatically equal the product le...
<p>(A) The p3B plasmid was linearized by PCR with primers p3Bf and p3Br. In parallel, the kanMX cass...
<p>(A) MluI (M), XhoI (X) and SalI (S) recognition sites are highlighted in green, blue and red, res...
<p>All reaction mixtures contained 1 mM ATP and 5 Weiss unit T4 DNA ligase (Fermentas) and incubated...
WOS: 000089634000029PubMed ID: 11310983This study compares a number of parameters that are important...
PCR fragments and linear vectors containing overlapping ends are easily assembled into a propagative...
<p>(<b>A</b>) Identification of positive and negative colonies by fluorescence. The circled colonies...
To research the effects of vector quantity and competence on the positive cloning rate, with a known...
<p>The number of colonies containing the correct constructs was counted for each experiment and the ...
The polymerase chain reaction (PCR) has found wide application in biochemistry and molecular biology...
A robust method for the in vivo cloning of large gene clusters was developed based on homologous rec...
(A) Transformation Efficiency comparison against the total concentration of DNA transformed for the ...
a<p>The competent cells [BL21(DE3)RIL] used had efficiency of 1×10<sup>8</sup> transformants/µg supe...
<div><p>A robust method for the <i>in vivo</i> cloning of large gene clusters was developed based on...
<p>(<b>A</b>) Schematic of SOC-mediated cycled-ligation assembly of multiple inserts directly and se...
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