Fig 2 -

(A) Transformation Efficiency comparison against the total concentration of DNA transformed for the indirect and direct cloning protocol in L. plantarum WCFS1 (The standard deviations correspond to three independent biological replicates) (B) Percentage accuracy of correct recombinant clones against the total concentration of DNA transformed in L. plantarum WCFS1 using the direct cloning protocol (The whiskers correspond to values from three independent biological replicates). (C) Agarose gel showing the colony PCR product (1140 bp) corresponding to the mCherry gene of interest (GOI). A red L. plantarum colony obtained after direct cloning was used as the template DNA for the PCR reaction. Generuler 100 bp Plus DNA Ladder (ThermoFisher ScientificTM) was used for the reference.