Effects of signal inhibitors and activators on cell proliferation and TER.

<p>(A) Effects of signal inhibitors and activators on the cell number in MDCK I cells. MDCK I cells were seeded at a density of 5.0 × 10⁴ cells/well in a 12-well plate, cultured for 48 h in the presence of the inhibitor or activator, and the cell number was counted with counting chamber after the trypsinization of the cells. (B) Effects of signal inhibitors and activators on the doubling time of MDCK I cells. The doubling time was calculated from the results in (A) on the assumption that the rate of cell proliferation was constant during the 48 h of the culture. (C) Effects of signal inhibitors and activators on TER. MDCK I cells were cultured on filters in the presence of the signal inhibitor or activator, and the TER was measured at each time point.