Effects of PI3K/Akt/mTOR and MAPK pathway inhibition on cell growth, proliferation, apoptosis and cell viability.

<p>RT112 and T24 cells were treated with RAD001 (5 nM), NVP-BEZ235 (100 nM for T24, 500 nM for RT112) and U0126 (25 nM) alone or the indicated combination and a DMSO control (ctr). <b>A:</b> For cell counts, cells were stained with trypan blue and numbers of living cells were determined. <b>B:</b> Cell cycle analysis after 24 h treatment with chemical compounds by BrdU incorporation in combination with 7-AAD staining. <b>C:</b> Measurement of caspase 3/7 activity as a parameter for apoptosis and <b>D:</b> XTT-test for detection of cell viability performed 24 h after treatment. Values shown are the mean ± standard deviation from 3 independent experiments. Student t-test was performed for statistic analysis (*: p<0,05).