LPC induced demyelination lesions in Prox-CKO-/- mutant mice had fewer OLs and greater NG2+ area.

<p>(A-C) Immunostaining with (A) BrdU, (B) NG2 and (C) CC1, in LPC-induced lesions in the spinal cords of <i>Prox1-CKO+/-</i> heterozygous (top) and <i>Prox1-CKO-/-</i> homozygous (bottom) mice, quantification in (D-F). Note that lesions have a higher nuclear (DAPI) density than surrounding tissue. (A,D) Fewer BrdU+ cells were observed within the lesions of <i>Prox1-CKO-/-</i> mutant mice. <i>Prox1-CKO+/-</i> n = 5 and <i>Prox1-CKO-/-</i> n = 4. (B,E) <i>Prox1-CKO-/-</i> lesions had increased NG2+ signal area. <i>Prox1-CKO+/-</i> n = 9 <i>and Prox1-CKO-/-</i> n = 8. (C,F) There were fewer CC1+ OLs within the lesions of <i>Prox1-CKO-/-</i> mice than in controls. <i>Prox1-CKO+/-</i> n = 8 and Prox1-/- n = 6. *** p<0.001;** p<0.01; * p<0.05 Student t-test; error bar: standard error of the mean. Dashed lines indicate the lesion boundary. Scale bar, 50 μm.