Extent of demyelination is decreased following NgR knock down.

<p>Coronal sections of adult mouse brains (12 µm) treated with saline, LPC and LPC+siNgR were double stained with MOG (green) and NF200 (red) and nuclei are labeled with DAPI (blue). (A) Schematic picture of injection site and demyelination area. (B) Saline-treated chiasm at 7 days post injection (dpi), no detectable demyelination is seen 7 days after a single injection of saline (Control). (C–E) Demyelination at optic chiasm of LPC-treated animals at 3, 7 and 14 dpi, respectively. (F–H) Demyelination at optic chiasm of LPC+siNgR treated animals at 3, 7 and 14 dpi, respectively. (I) The extent of demyelination in different groups are quantitatively analyzed and presented as percent of total area. Control group represents demyelination level in animals treated with saline at dpi 7. Statistical analysis used two-way ANOVA and Bonferroni's post -test. Treatment and time had a significant effect on the remyelination process. In LPC treated-animals significant demyelination is seen at 3, 7 and 14 dpi compared to saline (^***P<0.001). At 14 dpi, demyelination was partially reduced compared to 7 dpi. Between groups, there was a significant reduction of demyelination in LPC+siNgR 7 dpi compared to LPC 7 dpi (^∧∧∧P<0.001) and in LPC+siNgR 14 dpi compared to LPC 14 dpi (^∧∧∧P<0.001). Each data point shows data obtained from experiments carried out on three mice (n = 3), and represents Mean ± SEMs, Bars: 50 µm, Dashed line indicates lesion area.