Ara-C treatment caused a decrease in microglia, NG2+ progenitors, oligodendrocytes, astrocytes and T cells in the spinal cord of SOD1^G93A mice.

<p>Immunofluorescence for cell markers Iba1 (A), CD68 (B), NG2 (C), GFAP (D), CD3 (E) and Olig2 (F) in the lumbar spinal cord of mutant SOD1 transgenic mice treated with vehicle or Ara-C. (G) Quantification of Iba positive cells showed a 1.5 folds reduction of cells in Ara-C treated mice compared to controls (**<i>p</i> = 0.0086). (H) Quantification of CD68 marker showed a 1.5 folds reduction of cells in Ara-C treated mice compared to controls (**<i>p</i> = 0.0099). (I) Quantification of NG2+ marker showed a 1.7 folds reduction of cells in Ara-C treated mice compared to controls (<i>p</i> = 0.0713) (J) Quantification of GFAP marked cells showed a slightly reduced number of astrocytes (1.2 folds) in Ara-C treated mice compared to controls, although this result was not significant (<i>p</i> = 0.3430). (K) Quantification of Olig2 positive cells showed a 2.0 folds reduction of cells in Ara-C treated mice compared to controls (*<i>p</i> = 0.0236) (L) Quantification of CD3+ cells showed 3.8 folds reduction of cells in Ara-C treated mice compared to controls (*<i>p</i> = 0.0122). All mice were analyzed at 115 days. All values are means ± SEM. Scale bars: 100 µm.