Oral Administration of Polymyxin B Modulates the Activity of Lipooligosaccharide <i>E</i>. <i>coli</i> B against Lung Metastases in Murine Tumor Models - Fig 4

<p>A. Phenotypic characteristics of peritoneal macrophages. Peritoneal macrophages obtained from control or treated tumor bearing mice on 7^th, 14^th and 21^st day of experiment were stained in one-step test with following fluorophore-labeled monoclonal anti-mouse antibodies (mAbs): anti-F4/80-APC, anti-CD11b-PerCP-Cy5.5, anti-MHCII-FITC or anti-CD86-PE. Phenotypic characteristics of peritoneal macrophages obtained from healthy mice: CD11b^hiF4/80^hi – 67.23% (CD86 MFI—224, MHC II MFI—9), CD11b^loF4/80^lo – 11.22% (CD86 MFI—20, MHC II MFI 8) B. Cytokine production by peritoneal macrophages obtained from mice on 7 th day after <i>in vitro</i> LOS stimulation. The production of IL-6, IL-10 and TNF-α by stimulated (LOS 1μg/ml, 24h) peritoneal macrophages (1x10⁶ cells/ml) was evaluated by ELISA kits (BD Bioscience). In this supernatants, production of IL-4 was not observed. To calculate the mean four mice of each presented group were tested. Cytokine production by peritoneal macrophages obtained from healthy mice: IL-6–14.45±3.76 ng/ml, IL-10–3.58±1.13 ng/ml, IL-1β – 0.25±0.12 ng/ml. The relationship between groups was calculated for n>13 using Mann-Whitney <i>U</i> test. differences with a p<0.05 were regarded as significant (*p<0.05- calculated vs untreated mice, #p<0.05—calculated vs mice treated with PmB).