Dendritic cell (DC) activation is commonly used as a measure of the immunomodulatory potential of candidate exogenous and endogenous molecules. Residual lipopolysaccharide (LPS) contamination is a recurring theme and the potency of LPS is not always fully appreciated. To address this, polymyxin B (PmB) is often used to neutralise contaminating LPS. However, the limited capacity of this antibiotic to successfully block these effects is neglected. Therefore, this study aimed to determine the minimum LPS concentration required to induce murine bone marrow-derived dendritic cell (BMDC) maturation and cytokine secretion and to assess the ability of PmB to inhibit these processes. LPS concentrations as low as 10 pg/ml and 20 pg/ml induced secreti...
<p><b>A</b>) Example of how contaminating LPS in cytokine preparations can affect apoE production. T...
Very little is known about early events in LPS binding and about the duration of LPS exposure requir...
The immunostimulatory eVects of bacterial DNA on mammalian cells have been localized to unmethylated...
Dendritic cell (DC) activation is commonly used as a measure of the immunomodulatory potential of ca...
Dendritic cell (DC) activation is commonly used as a measure of the immunomodulatory potential of ca...
<p>BMDCs (6.25×10<sup>5</sup> cells/ml) from BALB/c mice were stimulated with concentrations from 1 ...
Dendritic cells have been described as effective antigen presenting cells. Human dentritic cells are...
Introduction: Polymyxin B (PmB) belongs to the group of cyclic peptide antibiotics, which neutralize...
The specific binding of radiolabeled polymyxin B (pmB) to rat alveolar macrophages was in-vestigated...
<p>A. Phenotypic characteristics of peritoneal macrophages. Peritoneal macrophages obtained from con...
<p>Monocytes were incubated for 8 h (TNF-α) or 24 h (IL-6 and CXCL8) with simplexide (10 µM) or LPS ...
Abstract Background Recombinant proteins expressed in Escherichia coli vectors are generally contami...
Polymyxin B (PMB) binds to and neutralizes endotoxin, but its systemic clinical utility is limited b...
Being one of the leading causes of death globally and especially in intensive care units, sepsis is ...
<p>ZYM-SIEC02 cells were stimulated with the indicated dose of <i>E.coli</i> 055:B55 LPS in the pres...
<p><b>A</b>) Example of how contaminating LPS in cytokine preparations can affect apoE production. T...
Very little is known about early events in LPS binding and about the duration of LPS exposure requir...
The immunostimulatory eVects of bacterial DNA on mammalian cells have been localized to unmethylated...
Dendritic cell (DC) activation is commonly used as a measure of the immunomodulatory potential of ca...
Dendritic cell (DC) activation is commonly used as a measure of the immunomodulatory potential of ca...
<p>BMDCs (6.25×10<sup>5</sup> cells/ml) from BALB/c mice were stimulated with concentrations from 1 ...
Dendritic cells have been described as effective antigen presenting cells. Human dentritic cells are...
Introduction: Polymyxin B (PmB) belongs to the group of cyclic peptide antibiotics, which neutralize...
The specific binding of radiolabeled polymyxin B (pmB) to rat alveolar macrophages was in-vestigated...
<p>A. Phenotypic characteristics of peritoneal macrophages. Peritoneal macrophages obtained from con...
<p>Monocytes were incubated for 8 h (TNF-α) or 24 h (IL-6 and CXCL8) with simplexide (10 µM) or LPS ...
Abstract Background Recombinant proteins expressed in Escherichia coli vectors are generally contami...
Polymyxin B (PMB) binds to and neutralizes endotoxin, but its systemic clinical utility is limited b...
Being one of the leading causes of death globally and especially in intensive care units, sepsis is ...
<p>ZYM-SIEC02 cells were stimulated with the indicated dose of <i>E.coli</i> 055:B55 LPS in the pres...
<p><b>A</b>) Example of how contaminating LPS in cytokine preparations can affect apoE production. T...
Very little is known about early events in LPS binding and about the duration of LPS exposure requir...
The immunostimulatory eVects of bacterial DNA on mammalian cells have been localized to unmethylated...