Oral Administration of Polymyxin B Modulates the Activity of Lipooligosaccharide <i>E</i>. <i>coli</i> B against Lung Metastases in Murine Tumor Models - Fig 3

<p>A. Mice treatment schedule. An administration of polymyxin B (7.38 mg/250 ml in drinking water) was started on the 7^th day before the i.v. injection of 2x10⁵ B16 cells and continued throughout the experiment duration. Mice were injected with LOS (1μg/mouse) i.p. one hour before cell injection. On the 7^th, 14^th and 21^st days after injection, material were harvested and number of lung metastases was determined. On the 7^th day metastases were not observed. B. Number of the B16 metastasis in the lung obtained from treated mice on the 14^th and 21^st day of experiment. To calculate the mean, 13–16 mice of each group of two experiments were tested. The relationship between groups was calculated for n>13 using Mann-Whitney <i>U</i> test. differences with a p<0.05 were regarded as significant (*p<0.05, **p<0.01—calculated vs untreated mice).