<p>Top. Representative RT-PCR products from <i>Dst</i>-<i>A1</i>, <i>-A2</i> and <i>-A3</i> with <i>actb</i> loading control in primary proliferating OPCs (left lanes) and differentiating OLs (right lanes). Bottom. qRT-PCR analysis of <i>Dst</i>-<i>A1</i>, <i>-A2</i> and <i>-A3</i> expression in primary proliferating OPCs and differentiating OLs. n = 4–6; ΔΔCt, <i>Dst</i> normalized to <i>actb</i>. * p<0.05, n.s. = p≥0.05; two-tailed Student’s t-test. Data represent mean ± SEM.</p
<p>(A–D, left) The mRNA of GAPDH (243 bp), KCNQ2 (172 bp), KCNQ3 (121 bp), KCNQ4 (110 bp) and KCNQ5 ...
<p>Neuronal cultures were exposed to siRNAs on days 3-5 in culture then exposed to various concentra...
Quantitative reverse transcriptase PCR (QRT-PCR) To determine the mRNA expression levels of Atf3, Bc...
<p>(A) phase contrast image (left) and fluorescence images (right) by marker MBP for OLs. The percen...
<p>RNA samples collected during differentiation at NP, E and L neuron stages for microarray studies ...
<p>(<b>A–B</b>) Results of the Neurogenesis and Neural Stem Cell PCR array. List of the genes up-reg...
<p>(A) Evidence of the neuronal-differentiated DPSC morphology change, bar scales 150 μm. (B) Semiqu...
A) The bar chart shows the number of detected circRNAs (>1 count/sample, see also Methods), comparin...
<p>(A) Representative RT-PCR data for S1PRs. Neurons constitutively expressed all S1PR subtypes rega...
A) Dot plots and bar graphs report the RT-qPCR results of the validation for Upf1 and Mettl3 selecte...
<p>Isolated NPCs were initially expanded and maintained in a culture medium containing EGF and bFGF ...
<p>(A) Flow cytometry analysis of cells co-stained with Tra-1-81 and SSEA4 antibodies in the three s...
<p>(<b>A</b>) qRT-PCR analysis of <i>FLVCR1a</i> mRNA levels in FLVCR1a-depleted SH-SY5Y cells compa...
<p>The term “isolated” is used to describe a glioma cell that is not in contact with other tumor cel...
This chapter highlights the use of the reverse transcription-polymerase chain reaction (RT-PCR) assa...
<p>(A–D, left) The mRNA of GAPDH (243 bp), KCNQ2 (172 bp), KCNQ3 (121 bp), KCNQ4 (110 bp) and KCNQ5 ...
<p>Neuronal cultures were exposed to siRNAs on days 3-5 in culture then exposed to various concentra...
Quantitative reverse transcriptase PCR (QRT-PCR) To determine the mRNA expression levels of Atf3, Bc...
<p>(A) phase contrast image (left) and fluorescence images (right) by marker MBP for OLs. The percen...
<p>RNA samples collected during differentiation at NP, E and L neuron stages for microarray studies ...
<p>(<b>A–B</b>) Results of the Neurogenesis and Neural Stem Cell PCR array. List of the genes up-reg...
<p>(A) Evidence of the neuronal-differentiated DPSC morphology change, bar scales 150 μm. (B) Semiqu...
A) The bar chart shows the number of detected circRNAs (>1 count/sample, see also Methods), comparin...
<p>(A) Representative RT-PCR data for S1PRs. Neurons constitutively expressed all S1PR subtypes rega...
A) Dot plots and bar graphs report the RT-qPCR results of the validation for Upf1 and Mettl3 selecte...
<p>Isolated NPCs were initially expanded and maintained in a culture medium containing EGF and bFGF ...
<p>(A) Flow cytometry analysis of cells co-stained with Tra-1-81 and SSEA4 antibodies in the three s...
<p>(<b>A</b>) qRT-PCR analysis of <i>FLVCR1a</i> mRNA levels in FLVCR1a-depleted SH-SY5Y cells compa...
<p>The term “isolated” is used to describe a glioma cell that is not in contact with other tumor cel...
This chapter highlights the use of the reverse transcription-polymerase chain reaction (RT-PCR) assa...
<p>(A–D, left) The mRNA of GAPDH (243 bp), KCNQ2 (172 bp), KCNQ3 (121 bp), KCNQ4 (110 bp) and KCNQ5 ...
<p>Neuronal cultures were exposed to siRNAs on days 3-5 in culture then exposed to various concentra...
Quantitative reverse transcriptase PCR (QRT-PCR) To determine the mRNA expression levels of Atf3, Bc...