DPSC neuronal and hepato differentiation characterization.

<p>(A) Evidence of the neuronal-differentiated DPSC morphology change, bar scales 150 μm. (B) Semiquantitative rt-PCR was used to determine the gene expression profiles ratio of neuronal specific markers in 1 month neuronal-differentiated over undifferentiated DPSC cells. Values were expressed as mean ± SD and were normalized using the β-actin. X-axis shows relative expression fold Y-axis tested markers, (adjusted <i>P</i><0.05). (C) FITC labeled antibody was used to evaluate, by IF, β³-tubulin expression in 1 month neuronal-differentiated DPSC, bar scales 75 μm. (D) FITC labeled antibody was used to evaluate, by IF, tyrosine hydroxylase expression in 1 month neuronal-differentiated DPSC, bar scales 50 μm. (E) FITC labeled antibody was used to evaluate, by IF, neurofilament-medium expression in 1 month neuronal-differentiated DPSC, bar scales 25 μm. (F) FITC labeled antibody was used to evaluate, by IF, glyal fibrillar acid protein expression in 1 month neuronal-differentiated DPSC, bar scales 75 μm. Nuclei were counter stained with DAPI. (G) Evidence of the hepato-differentiated DPSC morphology change, bar scales 150 μm. (H) Semiquantitative rt-PCR was used to determine the gene expression profiles ratio of hepatic specific markers in 40 days hepato-differentiated over undifferentiated DPSC cells. Values were expressed as mean ± SD and were normalized using the β-actin. X-axis shows relative expression fold Y-axis tested markers, (adjusted <i>P</i><0.05). (I) FITC labeled antibody was used to evaluate, by IF, cytokeratin 8 expression in 1 month hepato-differentiated DPSC, bar scales 75 μm. (J) FITC labeled antibody was used to evaluate, by IF, cytokeratin 18 expression in 1 month hepato-differentiated DPSC, bar scales 75 μm. (K) FITC labeled antibody was used to evaluate, by IF, cytokeratin 19 expression in 1 month hepato-differentiated DPSC, bar scales 75 μm. Nuclei were counter stained with DAPI. L: ELISA assay showed a time dependent increased albumin secretion in the medium from 11 ng/ml on day 25 to 25 ng/ml on day 31 after hepatic differentiation. Values were expressed as mean ± SD of three independent experiments, X-axis secreted albumin concentration (ng/ml) Y-differentiation days, (adjusted <i>P</i><0.05).