Neurons express all S1PR subtypes.

<p>(A) Representative RT-PCR data for S1PRs. Neurons constitutively expressed all S1PR subtypes regardless of oAβ–stimulation. (B) Immunostaining data for S1PRs in untreated neurons. Neurons constitutively expressed all S1PR subtypes. Scale bar, 20 µm. (C) Western blotting data for S1PRs. Arrowheads indicate the bands for S1PRs. Neurons constitutively expressed all S1PR subtypes regardless of oAβ–stimulation. NT, no treatment; oA 5 µM oAβ1-42 treatment. The positive control samples used for each S1PR were as follows; brain for S1P₁ and S1P₃; heart for S1P₂; spleen for S1P₄ and S1P₅.