Neural differentiation impacts on circRNAs biogenesis.

A) The bar chart shows the number of detected circRNAs (>1 count/sample, see also Methods), comparing pluripotent (ESC) and neural committed progenitors (NPC). Series of 4 Htt CAG expansion alleles (Q20, Q50, Q92 and Q111) are presented. Average circRNA count from two biological replicate experiments is plotted. B) Bars chart presents the circRNA fraction of total transcript mass in ESC and NPC different genotypes. C) The bars chart reports the percentage of circRNAs derived from specific transcripts areas: transcript coding sequence (CDS), 3’ or 5’ untranslated regions (3UTR/5UTR), whole transcript or other. The data compare ESC and NPC conditions. All Htt genotypes were combined. D) The box plot displays the spliced length distribution (in base pairs, bp) [average ± standard deviation (SD)] for circRNAs in the various conditions of ESC and NPC cells. Htt genotypes as in a). Wilcoxon test p-values of the difference between corresponding conditions of ESC and NPC are shown as stars (* (TIFF)