Exogenous and endogenous mFAM76B recognized by anti-hFAM76B MAbs.

<p>(A) Intranuclear localization of overexpressed mFAM76B in HEK 293 cells revealed by immunofluorescence staining. HEK 293 cells were transfected with plasmid expressing mFAM76B-Flag and stained with anti-hFAM76B MAbs and anti-Flag MAb. Normal mouse serum was used as a negative control. TRITC-conjugated goat anti-mouse IgG was used as the secondary antibody. Nuclei were labeled with DAPI (blue). (B) Endogenous mFAM76B revealed by immunohistochemical staining with MAbs against hFAM76B. NIH/3T3 and Hepa1-6 cells were fixed with 4% paraformaldehyde and then stained with the anti-hFAM76B MAbs. Normal mouse serum was used as a negative control. Primary Abs were detected by a Vectastain ABC Kit with DAB as a substrate. Nuclei were labeled with hematoxylin. Bar = 50 μm.