Cross binding of CD137L to CD137 between human and mouse species by ELISA assay.

<p>(A) Human or mouse CD137L-mFc (hL/mL) was coated (1 µg/ml) on an ELISA plate. After blocking with milk, supernatant containing the entire human or mouse CD137 extracellular domain (hE, mE) with human IgG tail was added and followed by HRP-goat-anti-human IgG for detection. (B) hL-mFc was coated to an ELISA plate and supernatants containing hE, hE4, mE, mE3 and mE4 all with h-Fc tail fusion proteins were added. hE and hE4 were used as positive controls and supernatant from cells that had not been transfected was used as a negative control. HRP-goat-anti-human IgG was added for detection. The figure shows representative results from one of three experiments. *P/N>2.1.