Immunofluoresence assay of the S0.7 and HSP70C proteins that were expressed in HEK 293 cells following transduction with rAd-GnS0.7-pCAG-HSP70C.

<p>HEK 293 cell antigen slides were prepared for detection of S0.7 and HSP70C. Mouse monoclonal antibody 1A8 or mouse anti-HSP70 antibody was used respectively to detect S0.7 or HSP70C. FITC-conjugated goat anti-mouse antibody was used to stain for positive fluorescence signals. Normal HEK 293 cells were used as negative controls. (A), S0.7 protein as detected with 1A8; (B), HSP70C protein as detected with anti-HSP70 antibody; and (C) normal HEK 293 cells as detected with a mixture of the above monoclonal antibodies.