Inhibition of growth by TPEN is rescued by the addition of Zn²⁺ ions.

<p>(<b>A</b>) Susceptibility to Zn²⁺ starvation of wild type, Δ<i>adcA</i> and Δ<i>lmb</i> null mutants grown in THY medium containing increasing concentrations of the chelating agent TPEN. (<b>B</b>) Inhibition of growth by 50 μM TPEN is rescued by the addition of either 50 μM Zn²⁺ in wild type as well as in Δ<i>adcA</i> and Δ<i>lmb</i> null mutants. Error bars represent the standard deviation.