Effect of Zn<sup>2+</sup> on activity of KDAC8.
- Tasha B. Toro (844368)
- Subramanya Pingali (844369)
- Thao P. Nguyen (844370)
- Destane S. Garrett (844371)
- Kyra A. Dodson (844372)
- Kyara A. Nichols (844373)
- Rashad A. Haynes (844374)
- Florastina Payton-Stewart (844375)
- Terry J. Watt (844376)
Publication date
January 2016
DOIAbstract
<p>KDAC8 was incubated with Fluor-de-Lys substrate in assay buffer 3 with 0-10X excess Zn<sup>2+</sup>, as detailed in the Experimental section. The rapid decline in activity indicates that even a small amount of excess zinc is binding to the low affinity inhibitory site, indicating active site saturation. Error bars represent the standard deviation of technical triplicates.</p
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