HDAC3:NCOR2 Inhibition by Zn(II).

HDAC3:NCOR2 (2 μM final concentration) was incubated with various concentrations of Zn(II) for one hour on ice and then diluted to 50 nM and assayed using the Fluor de Lys assay (panel A). Assay buffer was composed of 25 mM Tris, pH 8, and 10 mM KCl. The baseline sample buffer contained 500 μM EDTA and no added Zn(II). Observed rates at each concentration of Zn(II) were normalized to the baseline rate (panel B).