The importance of antioxidant genes to growth under zinc-limiting conditions.

<p>Wild type (WT, BY4743) cells and isogenic mutants defective for various antioxidant proteins were inoculated into LZM medium supplemented with 1000 µM (+Zn) or 1 µM (–Zn) ZnCl₂ at an initial optical density measured at 600 nm (OD₆₀₀) of 0.01 and 0.02, respectively. The cultures were then incubated at 30°C with aeration and their optical densities were determined after 40 h. A) Mutants defective in peroxiredoxins or other thioredoxin-related antioxidant proteins. B) Mutants defective in glutathione peroxidases or other glutathione-related antioxidant proteins. C) Mutants defective in superoxide dismutases, catalases, cytochrome c peroxidase, or oxidative stress-responsive transcription factors. The plotted values represent the means of three independent cultures and the <i>error bars</i> indicate 1 S.D.