Additional file 4: Figure S3. of Regulation of distinct branches of the non-canonical Wnt-signaling network in Xenopus dorsal marginal zone explants

All chimeras are translated into a protein of the expected size biologically active. In vitro transcribed and translated biotinylated proteins of the chimeric constructs were detected on a western blot via an AP conjugated Streptavidin antibody and visualized with NBT/BCIP. (A) The chimeras between the canonical xWnt8a and the non-canonical xWnt11 are translated in a protein of the expected size. (B) All non-canonical Wnt chimeras are translated in a protein of the expected size. (C) ATF2-luciferase reporter assay of HEK293 cells. All non-canonical chimera pairs are biologically active. Shown is the fold activation of the non-canonical ATF2-luciferase reporter of two independent sets of experiments. The differences in activation between the two sets of experiments are due to different batches of HEK293 cells. In both sets of experiments the chimeric constructs activate the ATF-luciferase reporter in a similar manner as wild-type Wnts. Thus, the chimeras are biologically active non-canonical Wnts. N: number of biological replicates, n: number of independent transfections; * P < 0.05, ** P < 0.01, *** P < 0.001 according to Student’s t test. (TIF 676 kb