Real-time quantitative PCR profile of <i>LmDEF</i> transcripts following <i>N</i>. <i>locustae</i> infection in the fat body (FB) and salivary glands (SG) in relation to time.

<p>Transcript accumulation values were normalized to the constitutively expressed β-actin gene and expressed as a function of the reference condition according to the 2^−ΔΔC_T method. The bars indicate the relative changes in RNA levels compared with the average expression of each gene under non-infected control conditions. The error bars indicate the standard errors of the means. The results are the means of at least three independent experiments. The statistical annotations, including the letters indicating significance, among treatments are given in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0161585#pone.0161585.s004" target="_blank">S4 Fig</a>.