Real-time PCR analysis of selected genes.

<p>Total RNA was extracted from the spleens of large yellow croakers sampled at 0, 12 and 24 h after poly(I:C) induction. Real-time PCR was used to validate gene expression changes in the pattern recognition receptors (A), signal transducers (B), interferons and interleukin (C), interferon-stimulated genes (D), JAK-STAT pathway (F), and T-cell receptor (TCR) signaling pathway. Increases and decreases in the relative levels of transcripts with respect to the control β-actin gene are shown.^*<i>P</i>< 0.05, ^**<i>P</i>< 0.01.