Quantitative RT-PCR demonstrates differential expression of salivary gland genes at 24 and 66 h of feeding.

<p>Relative quantitation of gene expression for selected set of salivary genes confirmed the observations made using the subset oligonucleotide array (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0000451#pone-0000451-t001" target="_blank">Table 1</a>). Fold change in gene expression at 66 h relative to 24 h. Error bars represent Mean fold change±SD. Expressions of most of the genes were increased at 66 h when compared to the levels at 24 h of feeding. The expression of at least 2 genes was increased at 24 h compared to that at 66 h (Inset) presented here as fold change in gene expression at 24 h relative to 66 h.