DHA inhibits ECV304 cell invasion by suppressing uPAR.

<p>(A) (B) Cells (10⁵) were incubated with 100 nM TPA in the presence or absence of 100 μM DHA or 200 ng/mL uPAR antibody or 200 ng/ml IgG antibody in a BIOCOAT^™Matrigel apparatus for 48 h. (C) Cells (10⁵) were incubated with 100 nM TPA in the presence of non-specific IgG (200 ng/mL), anti-uPAR antibody (200 ng/mL), 2 μM Ro, 20 μM PD, 20 μM SP, 20 μM Cur, and 20 μM BAY. After incubation, the cells invaded the undersurface of the chambers and were counted using a phase contrast light microscope after staining with a Diff-Quick Stain Kit. *<i>P</i><0.05 versus control; **<i>P</i><0.05 versus TPA only. The data represent the mean ± SD from triplicate measurements.