Effect of siRNA knockdown of the expression of NGF, TRKA, or p75^NTR on the ability of pancreatic cancer cells to migrate towards neurites extended from DRG.

<p>A) Representative images (top panel: bright field; bottom panel: green fluorescence) of the migration of Mia PaCa-2 cells towards nerves treated with siRNA oligonucleotides targeting the indicated genes. Arrows indicate the cancer cells that migrated towards the neurites. B) Quantification of the effect on the migration of cancer cells using relative invasion index. SF: siLentFect (transfection reagent), NT: Non-targeting siRNA control. ** ≤ 0.001; *** ≤ 0.0001 (compared to Non-targeting siRNA control).