Receptor specific silencing decreases NGF induced apoptosis.

<p>(A) A flow chart schematizing the siRNA experiments. (B) Confocal images depicting GFP positive cells (image) and p75^NTR-GFP double-positive cells (insert over the image), re-plated 5 hrs after electroporation. (C–D) Representative histogram for GFP specific staining, in comparison to control siRNA (control cells), showing a decreased p75^NTR positive expression and the related p75^NTR target gene deregulation. (E) Mean Fluorescence Intensity (MFI) specific for caspACE-VAD comparing 100 ng/mL NGF and 10 ng/mL TGFβ1 effects on control-siRNA, p75^NTR siRNA and double trkA^NGFR/p75^NTR siRNA treated cells. A 37.5%-decrease (*) and a 50%-decrease (**) of apoptotic signal were detected respectively in p75^NTR-siRNA and p75^NTR/trkA^NGFR-siRNA (p<.05).