BBSome-dependent recruitment of activated RAB-28 to the periciliary membrane.

<p><b>(A, B)</b> Representative images of amphid and phasmid cilia from N2 wild type (A) and <i>bbs-8(nx77)</i> mutant (B) worms expressing GFP-tagged RAB-28(WT), RAB-28(GDP) and RAB-28(GTP) reporters. All reporters are driven by the endogenous <i>rab-28</i> gene promoter. Kymographs and kymograph schematics derived from time-lapse imaging of GFP signals in phasmid cilia. Phenotypes summarised in cartoons. Large phasmid images are placed on black backgrounds. m; middle segment, d; distal segment. pcmc; periciliary membrane compartment (also denoted by arrow). Scale bars; 2 μm and 5 μm (low magnification phasmid images).