RAB-28 undergoes IFT.

<p><b>(A, B)</b> Representative images of phasmid cilia (left) from N2 wild type and <i>che-11(e1810)</i> worms expressing GFP::RAB-28, together with corresponding kymographs and kymograph schematics derived from time-lapse imaging. Distribution plot (and mean values) in B shows kymography-determined anterograde and retrograde GFP::RAB-28 velocities from wild type worms. MS; middle segment, DS; distal segment, PC; periciliary membrane compartment, DD; distal dendrite. Scale bars; 3 μm (phasmid image; and horizontal bar on kymographs); 3 seconds (vertical bar on kymographs). <b>(C)</b> Fluorescence recovery after photobleaching (FRAP) plots for GFP::RAB-28 in the phasmid neurons of N2 and <i>che-11(e1810)</i> mutant worms. GFP::RAB-28 also undergoes free diffusion in wild-type (N2) and <i>che-11</i> IFT mutant animals. Ciliary GFP signals bleached at time 0. Intensity measurements normalised to pre-bleach levels. Curves derived from 3 separate FRAP experiments. Error bars; SEM. Images taken from a representative FRAP experiment in N2 wild type worms. s; seconds. Scale bar; 2 μm.