RVVP and palmitoylation modification motifs prevent targeting of ARL-13 to ciliary distal segments and the nucleus.

<p>(<b>A–E</b>) Shown are worms expressing a GFP-tagged ARL-13 sequence variant alone (left-hand images) or together with a CHE-13/IFT57::mCherry transgene (right-hand cilium images). Note that CHE-13 ciliary levels are highly reduced in Δ285–370 and ΔRVVP variants. rPal; replacement of N-terminal palmitoylation modification motif cysteines with Ser-Ala <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1003977#pgen.1003977-Cevik1" target="_blank">[35]</a>. prs; proline-rich sequence. DS; distal segment. MS; middle segment. TZ; transition zone. BB; basal body. N; nucleus. Bars; 1 µm. (<b>F</b>) Plots of ARL-13 compartment length in phasmid cilia, at all larval stages, for worms expressing the indicated GFP-tagged ARL-13 variant or wild-type (WT) protein.