GFP-<i>Pf</i>Rab1A fluorescence is found as discrete loci in living cells.

<p>A. GFP fluorescence is observed as discrete loci termed <i>Pf</i>Rab1A bodies in trophozoite and schizont stage parasites expressing GFP-<i>Pf</i>Rab1A. B. GFP-<i>Pf</i>Rab18 fluorescence is diffuse and closely associated with the nuclei. C. C-terminal HA-tagged <i>Pf</i>Rab1A is distributed equally throughout the parasite cytoplasm. D. Infection by parasites expressing GFP-<i>Pf</i>Rab1A is less efficient than parasites expressing GFP-<i>Pf</i>Rab18 from the same promoter. Asterisks show significant differences for each time (p ≤ 0.01) using Student’s unpaired two-tailed t-test. E. While not associated with the nucleus, the number of GFP-<i>Pf</i>Rab1A loci is proportional to the number of nuclei in the cell.