Yeast two-hybrid interactions of five MADS-box B class proteins of <i>T</i>. <i>erecta</i>.

<p>The PCR products were introduced into the GAL4-based yeast two-hybrid vectors pGADT7 vector (AD) and pGBKT7 vector (BK) and co-transformed into the AH109 yeast strain. (a) Co-transformed yeast cells were plated on selection plates SD / -Trp-Leu-solid medium. (b) Yeast growth on selection plates SD / -Trp-Leu-His-Ade / X-α-GAL solid medium showed that TePI protein could interact with other four AP3/TM6-like proteins. No homodimer or interaction between the AP3/TM6-like proteins was observed in <i>T</i>. <i>erecta</i>. Besides, we observed homodimerization in TePI proteins.