hIPI3 interacts with some hORC and hMCM subunits in the yeast two-hybrid system.

<p>AH109 yeast host cells were co-transformed with the indicated combinations of plasmids and grown on SCM-Trp-Leu (SCM-2) plates. (A, B) Transformants were then patched onto SCM-2 and SCM-Trp-Leu-His (SCM-3) plates and incubated, to test cell growth. Cells containing AD-T (SV40 large T-antigen) and BD-p53 were used as the positive interaction control. Cells containing individual plasmids and their opposite empty vectors were included as negative controls. Positive interactions are marked by circles in the photos and shades on the tables. (C) A summary diagram of the relative strength of the protein-protein interactions based on the data shown in (A, B) and <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0151803#pone.0151803.s003" target="_blank">S3A and S3B Fig</a>. The symbols O1–O6 refer to hORC1-hORC6, and M2–M7 represent hMCM2-hMCM7. The thickness of the arrows indicates the observed relative strength of the interactions, with thin arrows representing weak interactions (recorded as “+” in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0151803#pone.0151803.s003" target="_blank">S3B Fig</a>) and the thicker arrows representing intermediate interactions (recorded as “++” in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0151803#pone.0151803.s003" target="_blank">S3B Fig</a>), relative to the strong interaction of the positive control (“+++”). Double arrows represent interactions detected in both directions, while single arrows indicate interactions detected in only one direction (eg. BD-hIPI3 interacts with AD-hORC5).